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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-19332 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FANCL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome.
Lagundžin D, Hu WF, Law HCH, Krieger KL, Qiao F, Clement EJ, Drincic AT, Nedić O, Naldrett MJ, Alvarez S, Woods NT
PloS one 2019;14(8):e0220568
PloS one 2019;14(8):e0220568
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Bone Marrow lysate using Product # PA5-19332 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Bone Marrow lysate using Product # PA5-19332 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 FA core complex assembly and FA protein expression in EndoC-betaH3 cells in response to glucose stimulation. A. Dot Plot of FA proteins identified in the FANCA co-IP analysis between 5 mM and 20 mM glucose conditions in EndoC-betaH3 cells. Dot size represents relative abundance. Dot color indicates the average number of spectra in each experiment. BFDR is represented by the outline shading. B. Expression of a panel of FA proteins in EndoC-betaH3 cells cultured in either 5 mM or 20 mM glucose for 1 hour determined by western blot. C. Confirmation of FANCB knockdown by shRNA (shFANCB) compared to non-targeting scrambled control shRNA (shScr) in EndoC-betaH3 cells. D. GSIS profiles in EndoC-betaH3 shScr or shFANCB transduced cells. Paired two-tailed Student's t-test p -values are displayed in the indicated comparisons above the graph. n = 3, mean +/- SEM, n.s. = not statistically significant. E. Intracellular insulin content of shScr or shFANCB EndoC-betaH3 cells used in the GSIS assays in panel C. Paired two-tailed Student's t-test p -values are displayed in the indicated comparisons above the graph. n = 3, mean +/- SEM.
