Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504711 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504711, RRID:AB_2622542
- Product name
- ACSF2 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)
- Antibody type
- Monoclonal
- Description
- ACSF2 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ACSF2
- Isotype
- IgG
- Antibody clone number
- OTI6E4
- Vial size
- 100 µl
- Concentration
- 0.53 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACSF2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ACSF2 mouse monoclonal antibody (TA504711) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSF2(RC204368).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ACSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504711)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504711)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204368 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACSF2 antibody(TA504711), and then analyzed by flow cytometry.
- Validation comment
- FC