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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- H00055198-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055198-B01P, RRID:AB_1571256
- Product name
- APPL2 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human APPL2 protein.
- Antigen sequence
MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTD
YTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEK
QNFALGKGDEEVISTLHYFSKVVDELNLLHTELAK
QLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEH
DLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQH
LSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINF
FKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAE
KMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQK
AGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRG
AVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGK
SGIILQAESRKENEEWICAINNISRQIYLTDNPEA
VAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNS
EMENENDKIVPKVTASLPEAEELIAPGTPIQFDIV
LPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLL
QQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARA
IHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFEL
TSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYI
FESNSEGEKICYAINLGKEIIEVQKDPEALAQLML
SIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The adaptor protein APPL1 increases glycogen accumulation in rat skeletal muscle through activation of the PI3-kinase signalling pathway.
Cleasby ME, Lau Q, Polkinghorne E, Patel SA, Leslie SJ, Turner N, Cooney GJ, Xu A, Kraegen EW
The Journal of endocrinology 2011 Jul;210(1):81-92
The Journal of endocrinology 2011 Jul;210(1):81-92
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of APPL2 expression in transfected 293T cell line (H00055198-T01) by APPL2 MaxPab polyclonal antibody.Lane 1: APPL2 transfected lysate(73.04 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- APPL2 MaxPab polyclonal antibody. Western Blot analysis of APPL2 expression in rat brain.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to APPL2 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
