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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-34730 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IP6K1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NCI-H929, human IHPK1-transfected 293T cells. Predicted reactivity: Mouse (97%), Rat (96%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IP6K1 using 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IP6K1 polyclonal antibody (Product # PA5-34730) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IP6K1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human IHPK1-transfected 293T cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IP6K1 polyclonal antibody (Product # PA5-34730) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IP6K1 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an IP6K1 polyclonal antibody (Product # PA5-34730) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
