orb97468

antibody from Biorbyt

Targeting: MAP1LC3B ATG8F

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

orb97468

Provider

Biorbyt

Product name

LC3 antibody

Antibody type

Monoclonal

Description

Mouse monoclonal antibody to LC3

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

5H12

Vial size

100 μl

Storage

Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C

Western blot

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Western blot analysis of human (AA: 1-125) recombinant protein using MAP1LC3B antibody

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Western blot analysis of HEK293 (Lane1) and MAP1LC3B (AA: 1-125)-hIgGFc transfected HEK293 (Lane2) cell lysate using MAP1LC3B antibody

ELISA

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of MAP1LC3B antibody

Flow cytometry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using MAP1LC3B antibody