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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB64842-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PILR-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human PILR-alpha in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q9UKJ1- Isotype
- IgG
- Antibody clone number
- 2175B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of PILR-alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human PILR-alpha or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PILR-alpha Monoclonal Antibody (Catalog # MAB64842) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PILR-alpha binding to PANP blocked by PILR-alpha Antibody. In a functional ELISA, Recombinant Human PILR-alpha (Catalog # 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 μg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-alpha Monoclonal Antibody (Catalog # MAB64842). At 1-5 μg/mL, this antibody will block 50% of the binding.
