PA5-30470

antibody from Invitrogen Antibodies

Targeting: ISCU hnifU, ISU2, NIFUN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30470

Provider

Invitrogen Antibodies

Product name

ISCU Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, ISCU-transfected 293T.

Concentration

0.66 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ISCU using 30 µg of HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ISCU polyclonal antibody (Product # PA5-30470) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ISCU was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a ISCU Polyclonal Antibody (Product # PA5-30470) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ISCU was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a ISCU Polyclonal Antibody (Product # PA5-30470) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ISCU was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a ISCU Polyclonal Antibody (Product # PA5-30470) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ISCU was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ISCU Polyclonal Antibody (Product # PA5-30470) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU (Product # PA5-30470) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ISCU Polyclonal Antibody detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ISCU Polyclonal Antibody (Product # PA5-30470) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.