Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB2189 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Carbonic Anhydrase X/CA10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Carbonic Anhydrase X/CA10 in ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9NS85
- Isotype
- IgG
- Antibody clone number
- 862319
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand.
Sterky FH, Trotter JH, Lee SJ, Recktenwald CV, Du X, Zhou B, Zhou P, Schwenk J, Fakler B, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):E1253-E1262
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):E1253-E1262
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Carbonic Anhydrase X/CA10 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1.5 µg/mL of Mouse Anti-Human Carbonic Anhydrase X/CA10 Monoclonal Antibody (Catalog # MAB2189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Carbonic Anhydrase X/CA10 at approximately 38-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.