MAB2189

antibody from R&D Systems

Targeting: CA10 CA-RPX, CARPX, HUCEP-15

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2189

Provider

R&D Systems

Product name

Human Carbonic Anhydrase X/CA10 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Carbonic Anhydrase X/CA10 in ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9NS85


Isotype

IgG

Antibody clone number

862319

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand.

Sterky FH, Trotter JH, Lee SJ, Recktenwald CV, Du X, Zhou B, Zhou P, Schwenk J, Fakler B, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 14;114(7):E1253-E1262

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Carbonic Anhydrase X/CA10 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1.5 µg/mL of Mouse Anti-Human Carbonic Anhydrase X/CA10 Monoclonal Antibody (Catalog # MAB2189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Carbonic Anhydrase X/CA10 at approximately 38-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.