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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM32014PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM32014PU-N, RRID:AB_11217185
- Product name
- anti POP7
- Antibody type
- Monoclonal
- Antigen
- Recombinant GST-hRPP20 (NCBI accession number AAC24113, expression vector pGEC-2T), expressed in E.coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F11
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Figure 1. Specificity of anti-hRPP20 Immunoglobulin, clone 1F11, determined by immunoblot analysis Blot contains total cell extract of HEp2 cells. Incubated with antibody fraction (0.5 mg/ml) diluted 500X in PBS containing 0,05% tween-20 and 5% non fat dry milk. Left: washing under stringent conditions. Right: washing under less stringent conditions.
