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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3083 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3083, RRID:AB_1574965
- Product name
- FRAT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FRAT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references FRAT1, a substrate-specific regulator of glycogen synthase kinase-3 activity, is a cellular substrate of protein kinase A.
Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of beta-catenin in ovarian tumours.
Casein kinase I epsilon enhances the binding of Dvl-1 to Frat-1 and is essential for Wnt-3a-induced accumulation of beta-catenin.
Hagen T, Cross DA, Culbert AA, West A, Frame S, Morrice N, Reith AD
The Journal of biological chemistry 2006 Nov 17;281(46):35021-9
The Journal of biological chemistry 2006 Nov 17;281(46):35021-9
Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of beta-catenin in ovarian tumours.
Wang Y, Hewitt SM, Liu S, Zhou X, Zhu H, Zhou C, Zhang G, Quan L, Bai J, Xu N
British journal of cancer 2006 Mar 13;94(5):686-91
British journal of cancer 2006 Mar 13;94(5):686-91
Casein kinase I epsilon enhances the binding of Dvl-1 to Frat-1 and is essential for Wnt-3a-induced accumulation of beta-catenin.
Hino S, Michiue T, Asashima M, Kikuchi A
The Journal of biological chemistry 2003 Apr 18;278(16):14066-73
The Journal of biological chemistry 2003 Apr 18;278(16):14066-73
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of FRAT1 (arrow) using rabbit FRAT1 polyclonal antibody (Cat # PAB3083). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAT1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FRAT1 polyclonal antibody (Cat # PAB3083) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
