NBP1-88534

antibody from Novus Biologicals

Targeting: MCU C10orf42, CCDC109A, FLJ46135

Provider product page for NBP1-88534

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [8]
Comments [0]
Validations
Western blot [4]
Immunohistochemistry [4]

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Product number: NBP1-88534 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-88534, RRID:AB_11017550
Product name: Rabbit Polyclonal CCDC109A Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. Specificity of human CCDC109A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

MCU protein structure - NBP1-88534 shown in red.

Submitted references Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter.

Lee Y, Min CK, Kim TG, Song HK, Lim Y, Kim D, Shin K, Kang M, Kang JY, Youn HS, Lee JG, An JY, Park KR, Lim JJ, Kim JH, Kim JH, Park ZY, Kim YS, Wang J, Kim DH, Eom SH
EMBO reports 2015 Oct;16(10):1318-33

Quantitative proteomics of synaptic and nonsynaptic mitochondria: insights for synaptic mitochondrial vulnerability.

Stauch KL, Purnell PR, Fox HS
Journal of proteome research 2014 May 2;13(5):2620-36

Reconstitution of the mitochondrial calcium uniporter in yeast.

Kovács-Bogdán E, Sancak Y, Kamer KJ, Plovanich M, Jambhekar A, Huber RJ, Myre MA, Blower MD, Mootha VK
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 17;111(24):8985-90

Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling.

Logan CV, Szabadkai G, Sharpe JA, Parry DA, Torelli S, Childs AM, Kriek M, Phadke R, Johnson CA, Roberts NY, Bonthron DT, Pysden KA, Whyte T, Munteanu I, Foley AR, Wheway G, Szymanska K, Natarajan S, Abdelhamed ZA, Morgan JE, Roper H, Santen GW, Niks EH, van der Pol WL, Lindhout D, Raffaello A, De Stefani D, den Dunnen JT, Sun Y, Ginjaar I, Sewry CA, Hurles M, Rizzuto R, UK10K Consortium., Duchen MR, Muntoni F, Sheridan E
Nature genetics 2014 Feb;46(2):188-93

Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity.

Arruda AP, Pers BM, Parlakgül G, Güney E, Inouye K, Hotamisligil GS
Nature medicine 2014 Dec;20(12):1427-35

Mitochondrial calcium uniporter Mcu controls excitotoxicity and is transcriptionally repressed by neuroprotective nuclear calcium signals.

Qiu J, Tan YW, Hagenston AM, Martel MA, Kneisel N, Skehel PA, Wyllie DJ, Bading H, Hardingham GE
Nature communications 2013;4:2034

The physiological role of mitochondrial calcium revealed by mice lacking the mitochondrial calcium uniporter.

Pan X, Liu J, Nguyen T, Liu C, Sun J, Teng Y, Fergusson MM, Rovira II, Allen M, Springer DA, Aponte AM, Gucek M, Balaban RS, Murphy E, Finkel T
Nature cell biology 2013 Dec;15(12):1464-72

Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter.

Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, Sancak Y, Bao XR, Strittmatter L, Goldberger O, Bogorad RL, Koteliansky V, Mootha VK
Nature 2011 Jun 19;476(7360):341-5

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: CCDC109A Antibody [NBP1-88534] - Simple Western lane view shows a specific band for MCU in 0.2 mg/ml of RT-4 (left), A549 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: CCDC109A Antibody [NBP1-88534] - Electropherogram image(s) of corresponding Simple Western lane view. CCDC109A antibody was used at 1:20 dilution on RT-4 and A549 lysate(s).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CCDC109A Antibody [NBP1-88534] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCU antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CCDC109A Antibody [NBP1-88534] - Analysis in control (vector only transfected HEK293T lysate) and MCU over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human hippocampus shows moderate to strong granular cytoplasmic positivity in neurons.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CCDC109A Antibody [NBP1-88534] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.