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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF6026 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal FACA/FANCA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human FACA/FANCA in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse FANCA, recombinant human (rh) FANCD2, rhFANCE, rhFANCF, rhFANCG, and rhFANCI is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Genomic amplification of Fanconi anemia complementation group A (FancA) in head and neck squamous cell carcinoma (HNSCC): Cellular mechanisms of radioresistance and clinical relevance.
Hess J, Unger K, Orth M, Schötz U, Schüttrumpf L, Zangen V, Gimenez-Aznar I, Michna A, Schneider L, Stamp R, Selmansberger M, Braselmann H, Hieber L, Drexler GA, Kuger S, Klein D, Jendrossek V, Friedl AA, Belka C, Zitzelsberger H, Lauber K
Cancer letters 2017 Feb 1;386:87-99
Cancer letters 2017 Feb 1;386:87-99
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human FACA/FANCA by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FACA/FANCA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6026) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FACA/FANCA at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
