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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- PAB10045 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10045, RRID:AB_1674802
- Product name
- FANCA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FANCA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair.
Identification of multiple nuclear export sequences in Fanconi anemia group A protein that contribute to CRM1-dependent nuclear export.
A novel duplication polymorphism in the FANCA promoter and its association with breast and ovarian cancer.
Smogorzewska A, Matsuoka S, Vinciguerra P, McDonald ER 3rd, Hurov KE, Luo J, Ballif BA, Gygi SP, Hofmann K, D'Andrea AD, Elledge SJ
Cell 2007 Apr 20;129(2):289-301
Cell 2007 Apr 20;129(2):289-301
Identification of multiple nuclear export sequences in Fanconi anemia group A protein that contribute to CRM1-dependent nuclear export.
Ferrer M, RodrÃguez JA, Spierings EA, de Winter JP, Giaccone G, Kruyt FA
Human molecular genetics 2005 May 15;14(10):1271-81
Human molecular genetics 2005 May 15;14(10):1271-81
A novel duplication polymorphism in the FANCA promoter and its association with breast and ovarian cancer.
Thompson E, Dragovic RL, Stephenson SA, Eccles DM, Campbell IG, Dobrovic A
BMC cancer 2005 Apr 29;5:43
BMC cancer 2005 Apr 29;5:43
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using FANCA polyclonal antibody (Cat # PAB10045) shows detection of FANCA only in FANCA transfected GM6914 cell lysates.No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection.Modified from Smogorzewska et al (2007) Cell 129, 289-301.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using FANCA polyclonal antibody (Cat # PAB10045) shows detection of a band at ~133 KDa (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates.The identity of the lower molecular weight bands is unknown, but may represent breakdown products.Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500.The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).IRDye™800 fluorescence images were captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
