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- References [1]
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- Immunohistochemistry [1]
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- Product number
- NBP1-03286 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-03286, RRID:AB_1520945
- Product name
- Rabbit Polyclonal FACA/FANCA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Amino acids 995-1009 of human FANCA protein.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
aa995-1009- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The Fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression.
Huard CC, Tremblay CS, Magron A, Lévesque G, Carreau M
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2152-7
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2152-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FACA/FANCA Antibody [NBP1-03286] - Anti-FANCA Antibody - Western Blot. Western blot using affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FACA/FANCA Antibody [NBP1-03286] - Anti-FANCA Antibody - Western Blot. Western blot using affinity purified anti-FANCA antibody shows detection of a band at ~133 kD (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FACA/FANCA Antibody [NBP1-03286] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
