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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-1343 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1343, RRID:AB_2164180
- Product name
- Goat Polyclonal Pleckstrin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Protein kinase C-mediated phosphorylation and activation of PDE3A regulate cAMP levels in human platelets.
Molecular cloning and expression of the major protein kinase C substrate of platelets.
Hunter RW, Mackintosh C, Hers I
The Journal of biological chemistry 2009 May 1;284(18):12339-48
The Journal of biological chemistry 2009 May 1;284(18):12339-48
Molecular cloning and expression of the major protein kinase C substrate of platelets.
Tyers M, Rachubinski RA, Stewart MI, Varrichio AM, Shorr RG, Haslam RJ, Harley CB
Nature 1988 Jun 2;333(6172):470-3
Nature 1988 Jun 2;333(6172):470-3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pleckstrin Antibody [NB100-1343] - Human PBMC lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody at 0.05 ug/mL, incubated for 1 hour. Detected by Western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pleckstrin Antibody [NB100-1343] - Staining of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pleckstrin Antibody [NB100-1343] - Staining of paraffin embedded Human Vessel (platelets). Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pleckstrin Antibody [NB100-1343] - Staining of paraffin embedded Human Spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Pleckstrin Antibody [NB100-1343] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
