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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP22534PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP22534PU-N, RRID:AB_10842210
- Product name
- anti Pleckstrin / PLEK (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide from C-term of human PLEK
- Reactivity
- Human
- Host
- Goat
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Antibody staining (0.05 µg/ml) of Human PBMC lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Vessel, Platelets (formalin-fixed, paraffin-embedded) stained with PLEK antibody AP22534PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Spleen (formalin-fixed, paraffin-embedded) stained with PLEK antibody AP22534PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
