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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-53234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: CPDVEFKRRF KEAFSKAAQQ TKGSYMEVED NRSQVETDDL ILKPGVVHVI DVDRGEEKKG KDQSGEVLSS V
- Concentration
- 0.30 mg/mL
Submitted references Engineered human blood-brain barrier microfluidic model for vascular permeability analyses.
Hajal C, Offeddu GS, Shin Y, Zhang S, Morozova O, Hickman D, Knutson CG, Kamm RD
Nature protocols 2022 Jan;17(1):95-128
Nature protocols 2022 Jan;17(1):95-128
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aquaporin 4 in human brain tissue using a Aquaporin 4 Polyclonal Antibody (Product # PA5-53234).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Aquaporin 4 in human cell line U-251 MG using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows localization to plasma membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Aquaporin 4 in mouse basal forebrain using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows positivity in a subset of astrocytes in the caudate putamen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human cerebral cortex using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows moderate to strong membranous positivity in astrocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human cerebellum using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows moderate to strong membranous positivity in astrocytes, mainly in the molecular layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human stomach using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows strong membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human liver using an Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows no positivity in hepatocytes, as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Aquaporin 4 in human cerebral cortex and liver tissues using Aquaporin 4 Polyclonal Antibody (Product # PA5-53234). Corresponding AQP4 RNA-seq data are presented for the same tissues.
