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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- GTX105971 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105971, RRID:AB_1952580
- Product name
- Wnt11 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references ERAD-dependent control of the Wnt secretory factor Evi.
Validating single-cell genomics for the study of renal development.
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, Christianson JC, Boutros M
The EMBO journal 2018 Feb 15;37(4)
The EMBO journal 2018 Feb 15;37(4)
Validating single-cell genomics for the study of renal development.
Jain S, Noordam MJ, Hoshi M, Vallania FL, Conrad DF
Kidney international 2014 Nov;86(5):1049-55
Kidney international 2014 Nov;86(5):1049-55
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ug whole cell lysate)A:Hep G2 (GTX27900)10% SDS PAGEGTX105971 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE GTX105971 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT11 antibody detects WNT11 protein by western blot analysis.A. 50 ?g mouse heart extract10% SDS-PAGEWNT11 antibody (GTX105971) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT11 antibody detects WNT11 protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g A431 whole cell extract C. 30 ?g HeLa whole cell extract D. 30 ?g HepG2 whole cell extract12% SDS-PAGEWNT11 antibody (GTX105971) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11(GTX105971) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT11 antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: WNT11 protein stained by WNT11 antibody (GTX105971) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11(GTX105971) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WNT11 antibody detects WNT11 protein at secreted on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. WNT11 antibody (GTX105971) dilution: 1:500.
