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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00005076-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005076-M01, RRID:AB_546781
- Product name
- Mouse Monoclonal Pax2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PAX2 - paired box gene 2
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex.
Human brain organoid-on-a-chip to model prenatal nicotine exposure.
Probing impaired neurogenesis in human brain organoids exposed to alcohol.
In situ generation of human brain organoids on a micropillar array.
Immunohistochemical markers of neural progenitor cells in the early embryonic human cerebral cortex.
Cerebral organoids model human brain development and microcephaly.
Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture.
Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells.
Naturally occurring variants of human aldo-keto reductases with reduced in vitro metabolism of daunorubicin and doxorubicin.
Patel D, Shimomura A, Majumdar S, Holley MC, Hashino E
PloS one 2018;13(1):e0191689
PloS one 2018;13(1):e0191689
Human brain organoid-on-a-chip to model prenatal nicotine exposure.
Wang Y, Wang L, Zhu Y, Qin J
Lab on a chip 2018 Mar 13;18(6):851-860
Lab on a chip 2018 Mar 13;18(6):851-860
Probing impaired neurogenesis in human brain organoids exposed to alcohol.
Zhu Y, Wang L, Yin F, Yu Y, Wang Y, Shepard MJ, Zhuang Z, Qin J
Integrative biology : quantitative biosciences from nano to macro 2017 Dec 11;9(12):968-978
Integrative biology : quantitative biosciences from nano to macro 2017 Dec 11;9(12):968-978
In situ generation of human brain organoids on a micropillar array.
Zhu Y, Wang L, Yu H, Yin F, Wang Y, Liu H, Jiang L, Qin J
Lab on a chip 2017 Aug 22;17(17):2941-2950
Lab on a chip 2017 Aug 22;17(17):2941-2950
Immunohistochemical markers of neural progenitor cells in the early embryonic human cerebral cortex.
Vinci L, Ravarino A, Fanos V, Naccarato AG, Senes G, Gerosa C, Bevilacqua G, Faa G, Ambu R
European journal of histochemistry : EJH 2016 Feb 1;60(1):2563
European journal of histochemistry : EJH 2016 Feb 1;60(1):2563
Cerebral organoids model human brain development and microcephaly.
Lancaster MA, Renner M, Martin CA, Wenzel D, Bicknell LS, Hurles ME, Homfray T, Penninger JM, Jackson AP, Knoblich JA
Nature 2013 Sep 19;501(7467):373-9
Nature 2013 Sep 19;501(7467):373-9
Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture.
Koehler KR, Mikosz AM, Molosh AI, Patel D, Hashino E
Nature 2013 Aug 8;500(7461):217-21
Nature 2013 Aug 8;500(7461):217-21
Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells.
Muguruma K, Nishiyama A, Ono Y, Miyawaki H, Mizuhara E, Hori S, Kakizuka A, Obata K, Yanagawa Y, Hirano T, Sasai Y
Nature neuroscience 2010 Oct;13(10):1171-80
Nature neuroscience 2010 Oct;13(10):1171-80
Naturally occurring variants of human aldo-keto reductases with reduced in vitro metabolism of daunorubicin and doxorubicin.
Bains OS, Grigliatti TA, Reid RE, Riggs KW
The Journal of pharmacology and experimental therapeutics 2010 Dec;335(3):533-45
The Journal of pharmacology and experimental therapeutics 2010 Dec;335(3):533-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Pax2 Antibody (3C7) [H00005076-M01] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of monoclonal antibody to PAX2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 0.5 ug/ml.
