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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6597 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6597, RRID:AB_1574286
- Product name
- DDX5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of DDX5.
- Antigen sequence
C-PMIGYPMPTGYSQ- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.
Iggo R, Gough A, Xu W, Lane DP, Spurr NK
Proceedings of the National Academy of Sciences of the United States of America 1989 Aug;86(16):6211-4
Proceedings of the National Academy of Sciences of the United States of America 1989 Aug;86(16):6211-4
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DDX5 polyclonal antibody (Cat # PAB6597) staining (0.5 ug/mL) of A-431 cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DDX5 polyclonal antibody (Cat # PAB6597, 2.5 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
