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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A302-420A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MED13L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED13L by western blot. Samples: Whole cell lysate (15 µg) from HeLa, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-MED13L antibody (Product # A302-420A; lot A302-420A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED13L by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MED13L antibody A302-420A (lot A302-420A-2) used for IP at 6 µg per reaction. MED13L was also immunoprecipitated by rabbit anti-MED13L antibody A302-421A. For blotting immunoprecipitated MED13L, A302-420A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED13L by western blot. Samples:Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED13L antibody A302-420A (lot A302-420A-2) used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED13L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody:Affinity purified rabbit anti-MED13L (Product # A302-420; lot 3) used at 1:1,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED13L by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- MED13L (Cat. No. A302-420A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MED13L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MED13L antibody (Product # A302-420A; lot A302-420A-3) used for IP at 6 µg per reaction. MED13L was also immunoprecipitated by a previous lot of this antibody (lot A302-420A-2) and rabbit anti-MED13L antibody (Product # A302-421A) For blotting immunoprecipitated MED13L (Product # A302-420A) was used at 0.04 µg/mL. Chemiluminescence with an exposure time of 30 seconds.
