Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-23491 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in Mouse NIH 3T3 cell lysate was probedusing a HMGB1 polyclonal antibody (Product # PA5-23491) at 5 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in mouse NIH 3T3 cell lysate. Samples were incubated in HMGB1 polyclonal antibody (Product # PA5-23491) using a dilution of 5 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in 0.2 mg/mL Jurkat lysate. Samples were incubated in HMGB1 polyclonal antibody (Product # PA5-23491). This experiment was performed under reducing conditions using the 12-230 kDa separation system.