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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-5286 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5286, RRID:AB_628411
- Product name
- Anti-TUBA1
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references The specificity of innate immune responses is enforced by repression of interferon response elements by NF-κB p50.
Depletion of Ku70/80 reduces the levels of extrachromosomal telomeric circles and inhibits proliferation of ALT cells.
ERG oncoprotein expression in prostate cancer: clonal progression of ERG-positive tumor cells and potential for ERG-based stratification.
Activation of FGF signaling mediates proliferative and osteogenic differences between neural crest derived frontal and mesoderm parietal derived bone.
Overexpression of microRNA-206 in the skeletal muscle from myotonic dystrophy type 1 patients.
Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice.
Parathyroid hormone accelerates decompensation following left ventricular hypertrophy.
Hematopoietic immortalizing function of the NKL-subclass homeobox gene TLX1.
Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning.
Nucleoporin phosphorylation triggered by the encephalomyocarditis virus leader protein is mediated by mitogen-activated protein kinases.
CpG-B oligodeoxynucleotides inhibit TLR-dependent and -independent induction of type I IFN in dendritic cells.
SUMOylation attenuates the function of PGC-1alpha.
Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects.
Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination.
Two novel functions of hyaluronidase-2 (Hyal2) are formation of the glycocalyx and control of CD44-ERM interactions.
CBP/p300 is a cell type-specific modulator of CLOCK/BMAL1-mediated transcription.
Hypoxic preconditioning results in increased motility and improved therapeutic potential of human mesenchymal stem cells.
Regulation of Survivin and CDK4 by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cell lines
Cheng CS, Feldman KE, Lee J, Verma S, Huang DB, Huynh K, Chang M, Ponomarenko JV, Sun SC, Benedict CA, Ghosh G, Hoffmann A
Science signaling 2011 Feb 22;4(161):ra11
Science signaling 2011 Feb 22;4(161):ra11
Depletion of Ku70/80 reduces the levels of extrachromosomal telomeric circles and inhibits proliferation of ALT cells.
Li B, Reddy S, Comai L
Aging 2011 Apr;3(4):395-406
Aging 2011 Apr;3(4):395-406
ERG oncoprotein expression in prostate cancer: clonal progression of ERG-positive tumor cells and potential for ERG-based stratification.
Furusato B, Tan SH, Young D, Dobi A, Sun C, Mohamed AA, Thangapazham R, Chen Y, McMaster G, Sreenath T, Petrovics G, McLeod DG, Srivastava S, Sesterhenn IA
Prostate cancer and prostatic diseases 2010 Sep;13(3):228-37
Prostate cancer and prostatic diseases 2010 Sep;13(3):228-37
Activation of FGF signaling mediates proliferative and osteogenic differences between neural crest derived frontal and mesoderm parietal derived bone.
Li S, Quarto N, Longaker MT
PloS one 2010 Nov 18;5(11):e14033
PloS one 2010 Nov 18;5(11):e14033
Overexpression of microRNA-206 in the skeletal muscle from myotonic dystrophy type 1 patients.
Gambardella S, Rinaldi F, Lepore SM, Viola A, Loro E, Angelini C, Vergani L, Novelli G, Botta A
Journal of translational medicine 2010 May 20;8:48
Journal of translational medicine 2010 May 20;8:48
Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Thèze J
The Journal of biological chemistry 2010 May 14;285(20):14898-908
The Journal of biological chemistry 2010 May 14;285(20):14898-908
Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice.
von Vietinghoff S, Asagiri M, Azar D, Hoffmann A, Ley K
Journal of immunology (Baltimore, Md. : 1950) 2010 Jul 1;185(1):670-8
Journal of immunology (Baltimore, Md. : 1950) 2010 Jul 1;185(1):670-8
Parathyroid hormone accelerates decompensation following left ventricular hypertrophy.
Cha H, Jeong HJ, Jang SP, Kim JY, Yang DK, Oh JG, Park WJ
Experimental & molecular medicine 2010 Jan 31;42(1):61-8
Experimental & molecular medicine 2010 Jan 31;42(1):61-8
Hematopoietic immortalizing function of the NKL-subclass homeobox gene TLX1.
Zweier-Renn LA, Hawley TS, Burkett S, Ramezani A, Riz I, Adler RL, Hickstein DD, Hawley RG
Genes, chromosomes & cancer 2010 Feb;49(2):119-31
Genes, chromosomes & cancer 2010 Feb;49(2):119-31
Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning.
Francis KR, Wei L
Cell death & disease 2010 Feb;1(2):e22
Cell death & disease 2010 Feb;1(2):e22
Nucleoporin phosphorylation triggered by the encephalomyocarditis virus leader protein is mediated by mitogen-activated protein kinases.
Porter FW, Brown B, Palmenberg AC
Journal of virology 2010 Dec;84(24):12538-48
Journal of virology 2010 Dec;84(24):12538-48
CpG-B oligodeoxynucleotides inhibit TLR-dependent and -independent induction of type I IFN in dendritic cells.
Liu YC, Gray RC, Hardy GA, Kuchtey J, Abbott DW, Emancipator SN, Harding CV
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 1;184(7):3367-76
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 1;184(7):3367-76
SUMOylation attenuates the function of PGC-1alpha.
Rytinki MM, Palvimo JJ
The Journal of biological chemistry 2009 Sep 18;284(38):26184-93
The Journal of biological chemistry 2009 Sep 18;284(38):26184-93
Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects.
Boon KL, Xiao S, McWhorter ML, Donn T, Wolf-Saxon E, Bohnsack MT, Moens CB, Beattie CE
Human molecular genetics 2009 Oct 1;18(19):3615-25
Human molecular genetics 2009 Oct 1;18(19):3615-25
Cdc42- and IRSp53-dependent contractile filopodia tether presumptive lens and retina to coordinate epithelial invagination.
Chauhan BK, Disanza A, Choi SY, Faber SC, Lou M, Beggs HE, Scita G, Zheng Y, Lang RA
Development (Cambridge, England) 2009 Nov;136(21):3657-67
Development (Cambridge, England) 2009 Nov;136(21):3657-67
Two novel functions of hyaluronidase-2 (Hyal2) are formation of the glycocalyx and control of CD44-ERM interactions.
Duterme C, Mertens-Strijthagen J, Tammi M, Flamion B
The Journal of biological chemistry 2009 Nov 27;284(48):33495-508
The Journal of biological chemistry 2009 Nov 27;284(48):33495-508
CBP/p300 is a cell type-specific modulator of CLOCK/BMAL1-mediated transcription.
Hosoda H, Kato K, Asano H, Ito M, Kato H, Iwamoto T, Suzuki A, Masushige S, Kida S
Molecular brain 2009 Nov 19;2:34
Molecular brain 2009 Nov 19;2:34
Hypoxic preconditioning results in increased motility and improved therapeutic potential of human mesenchymal stem cells.
Rosová I, Dao M, Capoccia B, Link D, Nolta JA
Stem cells (Dayton, Ohio) 2008 Aug;26(8):2173-82
Stem cells (Dayton, Ohio) 2008 Aug;26(8):2173-82
Regulation of Survivin and CDK4 by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cell lines
Mi Dan AI, Li Li LI, Xiao Rong ZHAO, Yong WU, Jian Ping GONG, Ya CAO
Cell Research 2005 Oct;15(10):777-784
Cell Research 2005 Oct;15(10):777-784
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 50
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
