LS-C500186

antibody from LSBio

Targeting: TUBA4A FLJ30169, H2-ALPHA, TUBA1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C500186

Provider

LSBio

Product name

TUBA4A / TUBA1 Antibody LS-C500186

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating TUBA4A in K562 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of TUBA4A Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: TUBA4A Antibody(4ug)+ K562 whole cell lysate(500ug);Lane 3: K562 whole cell lysate (20ug);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating TUBA4A in K562 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of TUBA4A Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: TUBA4A Antibody(4ug)+ K562 whole cell lysate(500ug);Lane 3: K562 whole cell lysate (20ug);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating TUBA4A in K562 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of TUBA4A Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: TUBA4A Antibody(4ug)+ K562 whole cell lysate(500ug);Lane 3: K562 whole cell lysate (20ug);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using TUBA4A Antibody at dilution of 1:100

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gastric cancer using TUBA4A Antibody at dilution of 1:100

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using TUBA4A Antibody at dilution of 1:100

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gastric cancer using TUBA4A Antibody at dilution of 1:100