H00007277-M01

antibody from Abnova Corporation

Targeting: TUBA4A FLJ30169, H2-ALPHA, TUBA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007277-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007277-M01, RRID:AB_437074

Product name

TUBA1 monoclonal antibody (M01), clone 2E11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant TUBA1.

Antigen sequence

MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQ
MPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDL
EPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYAR
GHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHS
FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAP
QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
DICRRNLDIERPTYTNLNRLISQIVSSITASLRFD
GALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMAC
CLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTG
FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIA
EAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGIDSYEDEDEGEE


Isotype

IgG

Antibody clone number

2E11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TUBA1 monoclonal antibody (M01), clone 2E11. Western Blot analysis of TUBA1 expression in HeLa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TUBA1 monoclonal antibody (M01), clone 2E11. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody (M01), clone 2E11.Lane 1: TUBA1 transfected lysate(49.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol