Ab088473

antibody from Aladdin Scientific Corporation

Targeting: TUBA4A FLJ30169, H2-ALPHA, TUBA1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

Ab088473

Provider

Aladdin Scientific Corporation

Product name

Recombinant Alpha Tubulin Antibody

Antibody type

Recombinant

Description

Rabbit anti Human Alpha Tubulin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.Post-translationalSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Reactivity

Human, Mouse, Rat, Hamster

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 400-448


Vial size

100_l,10_l,1ml,50_l

Concentration

0,25 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.