Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9009-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Noggin Monoclonal Antibody (1H8noggin), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 1H8noggin recognizes human noggin. Noggin is a secreted, glycosylated, chemokine that binds and inhibits TGFbeta (transforming growth factor beta) family members such as bone morphogenic protein-4 and -2 (BMP-4, BMP-2). The binding of noggin to BMPs prevents interaction with signal-transducing BMP receptors (BMPR), responsible for activation of VEGF and other pathways that affect vascular patterning, neural tube formation, mesodermal differentiation, and oligodendrogliogenesis. Noggin expression is restricted both temporally and by cell type, for example by chondrogenic precursor cells during limb development, ectodermal cells in the dorsal neural tube, ependymal cells adjacent to the subventricular zone in adult brain, and stromal cells underlying luminal epithelium. Applications Reported: This 1H8noggin antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This 1H8noggin antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 10 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1H8noggin
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Enhancement of human adipose-derived stromal cell angiogenesis through knockdown of a BMP-2 inhibitor.
Remyelination is altered by bone morphogenic protein signaling in demyelinated lesions.
Novel functions of Noggin proteins: inhibition of Activin/Nodal and Wnt signaling.
Levi B, Nelson ER, Hyun JS, Glotzbach JP, Li S, Nauta A, Montoro DT, Lee M, Commons GC, Hu S, Wu JC, Gurtner GC, Longaker MT
Plastic and reconstructive surgery 2012 Jan;129(1):53-66
Plastic and reconstructive surgery 2012 Jan;129(1):53-66
Remyelination is altered by bone morphogenic protein signaling in demyelinated lesions.
Sabo JK, Aumann TD, Merlo D, Kilpatrick TJ, Cate HS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Mar 23;31(12):4504-10
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Mar 23;31(12):4504-10
Novel functions of Noggin proteins: inhibition of Activin/Nodal and Wnt signaling.
Bayramov AV, Eroshkin FM, Martynova NY, Ermakova GV, Solovieva EA, Zaraisky AG
Development (Cambridge, England) 2011 Dec;138(24):5345-56
Development (Cambridge, England) 2011 Dec;138(24):5345-56
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human lung tissue stained with 10 µg/mL Mouse IgG2b K Isotype Control Purified (Product # 14-4732-82) (left) or 10 µg/mL Anti-Human Noggin Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP and DAB visualization.Nuclei are counterstained with hematoxylin.