Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00002707-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002707-M01, RRID:AB_2263389
- Product name
- Mouse Monoclonal Connexin 31/GJB3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GJB3 - gap junction protein, beta 3, 31kDa (connexin 31)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references EKV mutant connexin 31 associated cell death is mediated by ER stress.
Tattersall D, Scott CA, Gray C, Zicha D, Kelsell DP
Human molecular genetics 2009 Dec 15;18(24):4734-45
Human molecular genetics 2009 Dec 15;18(24):4734-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.Lane 1: GJB3 transfected lysate(31 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - GJB3 monoclonal antibody (M01), clone 3B4-1B3 Analysis of GJB3 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Detection limit for recombinant GST tagged GJB3 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of monoclonal antibody to GJB3 on HeLa cell. Antibody concentration 10 ug/ml.