711756

antibody from Invitrogen Antibodies

Targeting: AGO3 EIF2C3, FLJ12765, hAGO3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711756

Provider

Invitrogen Antibodies

Product name

Argonaute 3 Recombinant Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat, Sheep, and Bovine.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), U-2 OS (Lane 2), K562 (Lane 3) and HEL 92.1.7 (Lane 4). The blots were probed with Anti-AGO3 Recombinant Rabbit Polyclonal Antibody (Product # 711756, 1 µg/mL). A 110 kDa band corresponding to AGO3 was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).