Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 702721 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Argonaute 3 Recombinant Rabbit Monoclonal Antibody (22H29L11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Mouse, Rat, Bovine.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 22H29L11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Argonaute 3 Recombinant Rabbit Monoclonal Antibody (Product # 702721) and a 97 kDa band corresponding to Argonaute 3 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of T-47D (Lane 1), MCF7 (Lane 2), HeLa (Lane 3), Caco-2 (Lane 4), HEK-293 (Lane 5), Hep G2 (Lane 6) and HCT 116 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).