GTX48488

antibody from GeneTex

Targeting: ING3 Eaf4, FLJ20089, MEAF4, p47ING3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

GTX48488

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48488, RRID:AB_11170100

Product name

ING3 Antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Goat

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex's purified anti-ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 µg of RKO whole cell lysate (ATCC

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex's purified anti-p47 ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 ?g of RKO whole cell lysate separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4¢X C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] MXHur for 45 min at room temperature. IRDye?800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.