Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 17096-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17096-1-AP, RRID:AB_10598479
- Product name
- SPHK2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SPHK2 antibody (Cat. #17096-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor TĀ cell response.
Inhibition of sphingosine kinaseĀ 2 attenuates hypertrophic scar formation via upregulation of Smad7 in human hypertrophic scar fibroblasts.
Analysis of selective target engagement by small-molecule sphingosine kinase inhibitors using the Cellular Thermal Shift Assay (CETSA).
Mechanism of sphingosine 1-phosphate clearance from blood.
Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.
The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.
Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.
Novel TRRAP mutation causes autosomal dominant non-syndromic hearing loss.
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.
Flotillin proteins recruit sphingosine to membranes and maintain cellular sphingosine-1-phosphate levels.
Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.
Targeting sphingosine kinase 1 induces MCL1-dependent cell death in acute myeloid leukemia.
Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.
An oncogenic role for sphingosine kinase 2.
ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.
Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.
Vaena S, Chakraborty P, Lee HG, Janneh AH, Kassir MF, Beeson G, Hedley Z, Yalcinkaya A, Sofi MH, Li H, Husby ML, Stahelin RV, Yu XZ, Mehrotra S, Ogretmen B
Cell reports 2021 May 4;35(5):109076
Cell reports 2021 May 4;35(5):109076
Inhibition of sphingosine kinaseĀ 2 attenuates hypertrophic scar formation via upregulation of Smad7 in human hypertrophic scar fibroblasts.
Zeng J, Jiang B, Xiao X, Zhang R
Molecular medicine reports 2020 Sep;22(3):2573-2582
Molecular medicine reports 2020 Sep;22(3):2573-2582
Analysis of selective target engagement by small-molecule sphingosine kinase inhibitors using the Cellular Thermal Shift Assay (CETSA).
Hengst JA, Dick TE, Smith CD, Yun JK
Cancer biology & therapy 2020 Sep 1;21(9):841-852
Cancer biology & therapy 2020 Sep 1;21(9):841-852
Mechanism of sphingosine 1-phosphate clearance from blood.
Kharel Y, Huang T, Salamon A, Harris TE, Santos WL, Lynch KR
The Biochemical journal 2020 Mar 13;477(5):925-935
The Biochemical journal 2020 Mar 13;477(5):925-935
Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.
Blais-Lecours P, Laouafa S, Arias-Reyes C, Santos WL, Joseph V, Burgess JK, Halayko AJ, Soliz J, Marsolais D
American journal of respiratory cell and molecular biology 2020 Jan;62(1):35-42
American journal of respiratory cell and molecular biology 2020 Jan;62(1):35-42
The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.
Li J, Zhao LM, Zhang C, Li M, Gao B, Hu XH, Cao J, Wang GY
Oncology research 2020 Feb 7;28(1):51-63
Oncology research 2020 Feb 7;28(1):51-63
Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.
Yu J, Dong J, Chen K, Ding Y, Yang Z, Lan T
Transgenic research 2020 Aug;29(4):419-428
Transgenic research 2020 Aug;29(4):419-428
Novel TRRAP mutation causes autosomal dominant non-syndromic hearing loss.
Xia W, Hu J, Ma J, Huang J, Wang X, Jiang N, Zhang J, Ma Z, Ma D
Clinical genetics 2019 Oct;96(4):300-308
Clinical genetics 2019 Oct;96(4):300-308
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
Xiao J, Lin H, Liu B, Xia Z, Zhang J, Jin J
Biomarkers in medicine 2019 Jun;13(8):627-637
Biomarkers in medicine 2019 Jun;13(8):627-637
Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.
Neubauer HA, Tea MN, Zebol JR, Gliddon BL, Stefanidis C, Moretti PAB, Pitman MR, Costabile M, Kular J, Stringer BW, Day BW, Samuel MS, Bonder CS, Powell JA, Pitson SM
Oncogene 2019 Feb;38(8):1151-1165
Oncogene 2019 Feb;38(8):1151-1165
Flotillin proteins recruit sphingosine to membranes and maintain cellular sphingosine-1-phosphate levels.
Riento K, Zhang Q, Clark J, Begum F, Stephens E, Wakelam MJ, Nichols BJ
PloS one 2018;13(5):e0197401
PloS one 2018;13(5):e0197401
Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.
Panneer Selvam S, Roth BM, Nganga R, Kim J, Cooley MA, Helke K, Smith CD, Ogretmen B
The Journal of biological chemistry 2018 Jun 22;293(25):9784-9800
The Journal of biological chemistry 2018 Jun 22;293(25):9784-9800
Targeting sphingosine kinase 1 induces MCL1-dependent cell death in acute myeloid leukemia.
Powell JA, Lewis AC, Zhu W, Toubia J, Pitman MR, Wallington-Beddoe CT, Moretti PA, Iarossi D, Samaraweera SE, Cummings N, Ramshaw HS, Thomas D, Wei AH, Lopez AF, D'Andrea RJ, Lewis ID, Pitson SM
Blood 2017 Feb 9;129(6):771-782
Blood 2017 Feb 9;129(6):771-782
Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.
Neubauer HA, Pitson SM
F1000Research 2016;5:2825
F1000Research 2016;5:2825
An oncogenic role for sphingosine kinase 2.
Neubauer HA, Pham DH, Zebol JR, Moretti PA, Peterson AL, Leclercq TM, Chan H, Powell JA, Pitman MR, Samuel MS, Bonder CS, Creek DJ, Gliddon BL, Pitson SM
Oncotarget 2016 Oct 4;7(40):64886-64899
Oncotarget 2016 Oct 4;7(40):64886-64899
ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.
Liu X, Ren K, Suo R, Xiong SL, Zhang QH, Mo ZC, Tang ZL, Jiang Y, Peng XS, Yi GH
Journal of physiology and biochemistry 2016 Dec;72(4):657-667
Journal of physiology and biochemistry 2016 Dec;72(4):657-667
Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.
Sanchez T, Estrada-Hernandez T, Paik JH, Wu MT, Venkataraman K, Brinkmann V, Claffey K, Hla T
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP(SPHK2 antibody) at dilution of 1:300
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 17096-1-AP(SPHK2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPHK2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPHK2. This antibody recognizes human,mouse,rat antigen. The SPHK2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.