H00056848-D01

antibody from Abnova Corporation

Targeting: SPHK2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00056848-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00056848-D01, RRID:AB_10713713

Product name

SPHK2 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human SPHK2 protein.

Antigen sequence

MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAK
AMAPPPPPLAASTPLLHGEFGSYPARGPRFALTLT
SQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRS
RSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGA
ATYEENRAEAQRWATALTCLLRGLPLPGDGEITPD
LLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISE
AGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVS
GDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGN
ALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHP
LDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERF
RALGSARFTLGTVLGLATLHTYRGRLSYLPATVEP
ASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSV
SDLPLPLPQPALASPGSPEPLPILSLNGGGPELAG
DWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGA
PVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGT
PLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHAR
FDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSL
GCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGP
LQAQMHPGIGTLLTGPPGCPGREP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SPHK2 expression in transfected 293T cell line (H00056848-T01) by SPHK2 MaxPab polyclonal antibody.Lane 1: SPHK2 transfected lysate(69.20 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of SPHK2 transfected lysate using anti-SPHK2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SPHK2 purified MaxPab mouse polyclonal antibody (B01P) (H00056848-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol