Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 703636 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Alpha-2a Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody (3H29L10)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Cat, Pig, Mouse.
- Antibody clone number
- 3H29L10
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, HEK-293 cells were fixed and permeabilized for detection of endogenous Alpha-2a Adrenergic Receptor using Anti-Alpha-2a Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody (Product # 703636, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative control cells that were stained for detection and localization of Alpha-2a Adrenergic Receptor protein (green) with reduced signal compared to panel e-h) clearly demonstrating enhanced cytoplasmic and membrane localisation of Alpha-2a Adrenergic Receptor in serum-starved (48h) HEK-293 cells. The images were captured at 60X magnification.