Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 321305 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#321305, RRID:AB_492899
- Product name
- FITC anti-human/mouse Cutaneous Lymphocyte Antigen (CLA)
- Antibody type
- Monoclonal
- Antigen
- Cutaneous Lymphocyte Antigen (CLA)
- Description
- Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is suitable for staining formalin-fixed paraffin embedded tissue sections.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgM
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Granulomatosis with polyangiitis involves sustained mucosal inflammation that is rich in B-cell survival factors and autoantigen.
Improvement of psoriasis after tonsillectomy is associated with a decrease in the frequency of circulating T cells that recognize streptococcal determinants and homologous skin determinants.
Zhao Y, Odell E, Choong LM, Barone F, Fields P, Wilkins B, Tungekar FM, Patel P, Sanderson JD, Sangle S, D'Cruz D, Spencer J
Rheumatology (Oxford, England) 2012 May 23;
Rheumatology (Oxford, England) 2012 May 23;
Improvement of psoriasis after tonsillectomy is associated with a decrease in the frequency of circulating T cells that recognize streptococcal determinants and homologous skin determinants.
Thorleifsdottir RH, Sigurdardottir SL, Sigurgeirsson B, Olafsson JH, Sigurdsson MI, Petersen H, Arnadottir S, Gudjonsson JE, Johnston A, Valdimarsson H
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):5160-5
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):5160-5
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with HECA-452 FITC
- Conjugate
- Green dye
