LS-C354948

antibody from LSBio

Targeting: CYLD CYLD1, KIAA0849, USPL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C354948

Provider

LSBio

Product name

CYLD Antibody (N-Terminus, clone 733) LS-C354948

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human, Mouse, Rat, Bovine, Horse, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

733

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

WB using CYLD Antibody (733)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYLD showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CYLD Mouse Monoclonal antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.