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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009457-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009457-M01, RRID:AB_425808
- Product name
- FHL5 monoclonal antibody (M01), clone 1G12-D2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant FHL5.
- Antigen sequence
MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDR
VFSNYCEECKKPIESDSKDLCYKDRHWHEGCFKCT
KCNHSLVEKPFAAKDERLLCTECYSNECSSKCFHC
KRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTK
PLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGI
TFCDQLWHKECFLCSDCRKDLCEEQFMSRDDYPFC
MDCYNHLYANKCVACSKPISGLTGAKFICFQDSQW
HSECFNCGKCSVSLVGKGFLTQNKEIFCQKCGSGM
DTDI- Isotype
- IgG
- Antibody clone number
- 1G12-D2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2.Lane 1: FHL5 transfected lysate(32.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to FHL5 on HepG2 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
