14025-1-AP

antibody from Proteintech Group

Targeting: SOCS3 CIS3, Cish3, SOCS-3, SSI-3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14025-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14025-1-AP, RRID:AB_10597854

Product name

SOCS3 antibody

Antibody type

Polyclonal

Description

KD/KO validated SOCS3 antibody (Cat. #14025-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.

Tian D, Teng X, Jin S, Chen Y, Xue H, Xiao L, Wu Y
Journal of advanced research 2021 Jan;27:115-125

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Pro-protein convertase subtilisin/kexin type 9 promotes intestinal tumor development by activating Janus kinase 2/signal transducer and activator of transcription 3/SOCS3 signaling in Apc(Min/+) mice.

Yang K, Zhu J, Luo HH, Yu SW, Wang L
International journal of immunopathology and pharmacology 2021 Jan-Dec;35:20587384211038345

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GCSF deficiency attenuates nonalcoholic fatty liver disease through regulating GCSFR-SOCS3-JAK-STAT3 pathway and immune cells infiltration.

Zhang Y, Zhou X, Liu P, Chen X, Zhang J, Zhang H, Li S, Chen Y, Song X, Wang J, Zeng H, Zhang X, Tang C, Yu C, Li Y, Xu C
American journal of physiology. Gastrointestinal and liver physiology 2021 Apr 1;320(4):G531-G542

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Modulation of Wnt/β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells.

Yuan C, Yang D, Ma J, Yang J, Xue J, Song F, Liu X
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2020;53(8):e9488

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Knockdown of m6A methyltransferase METTL3 in gastric cancer cells results in suppression of cell proliferation.

Jiang L, Chen T, Xiong L, Xu JH, Gong AY, Dai B, Wu G, Zhu K, Lu E, Mathy NW, Chen XM
Oncology letters 2020 Sep;20(3):2191-2198

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Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Ebokaiwe AP, Njoya EM, Sheng Y, Zhang Z, Li S, Zhou Z, Qiang Z, Peng T, Hussein AA, Zhang G, Lu X, Li L, Wang F
Toxicology and applied pharmacology 2020 Oct 1;404:115203

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Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Cai BB, Lu YN, Xu M
Acta pharmacologica Sinica 2020 May;41(5):650-660

---

gga-miR-27b-3p enhances type I interferon expression and suppresses infectious bursal disease virus replication via targeting cellular suppressors of cytokine signaling 3 and 6 (SOCS3 and 6).

Duan X, Zhao M, Li X, Gao L, Cao H, Wang Y, Zheng SJ
Virus research 2020 May;281:197910

---

Long non-coding RNA expression profiling following treatment with resveratrol to improve insulin resistance.

Shu L, Hou G, Zhao H, Huang W, Song G, Ma H
Molecular medicine reports 2020 Aug;22(2):1303-1316

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Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.

Shiau DJ, Kuo WT, Davuluri GVN, Shieh CC, Tsai PJ, Chen CC, Lin YS, Wu YZ, Hsiao YP, Chang CP
Scientific reports 2020 Aug 12;10(1):13582

---

Expression and methylation levels of suppressor of cytokine signaling 3 in rheumatic arthritis synovial fibroblasts.

Meng C, Qi H, Wang X, Wu X, Huang W, Wang H, He Y
Experimental and molecular pathology 2020 Apr;113:104361

---

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Wang D, Xu B, Wang J, Wang H, Guo J, Ji H, Li S, Wu R, Yang H, Lian S
Brain research 2019 Nov 1;1722:146354

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Adeno-associated virus 9-mediated RNA interference targeting SOCS3 alleviates diastolic heart failure in rats.

Gao J, Guo Y, Chen Y, Zhou J, Liu Y, Su P
Gene 2019 May 20;697:11-18

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Exosome-Mediated Delivery of Inducible miR-423-5p Enhances Resistance of MRC-5 Cells to Rabies Virus Infection.

Wang J, Teng Y, Zhao G, Li F, Hou A, Sun B, Kong W, Gao F, Cai L, Jiang C
International journal of molecular sciences 2019 Mar 27;20(7)

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Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1.

Zhao G, Gong L, Su D, Jin Y, Guo C, Yue M, Yao S, Qin Z, Ye Y, Tang Y, Wu Q, Zhang J, Cui B, Ding Q, Huang H, Hu L, Chen Y, Zhang P, Hu G, Chen L, Wong KK, Gao D, Ji H
The Journal of clinical investigation 2019 Mar 1;129(3):972-987

---

Low doses of decitabine improve the chemotherapy efficacy against basal-like bladder cancer by targeting cancer stem cells.

Wu M, Sheng L, Cheng M, Zhang H, Jiang Y, Lin S, Liang Y, Zhu F, Liu Z, Zhang Y, Zhang X, Gao Q, Chen D, Li J, Li Y
Oncogene 2019 Jul;38(27):5425-5439

---

High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.

Wang J, Gao WJ, Deng SL, Liu X, Jia H, Ma WZ
Stem cell research & therapy 2019 Jul 29;10(1):227

---

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.

Zhu Y, Meng Y, Zhao Y, Zhu J, Xu H, Zhang E, Shi L, Du L, Liu G, Zhang C, Xu X, Kang X, Zhen Y, Zhang S
Colloids and surfaces. B, Biointerfaces 2019 Jul 1;179:66-76

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m⁶A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Wu R, Liu Y, Zhao Y, Bi Z, Yao Y, Liu Q, Wang F, Wang Y, Wang X
Cell death & disease 2019 Feb 20;10(3):171

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Neonatal Overnutrition Increases Testicular Size and Expression of Luteinizing Hormone β-Subunit in Peripubertal Male Rats.

Argente-Arizón P, Castro-González D, Díaz F, Fernández-Gómez MJ, Sánchez-Garrido MA, Tena-Sempere M, Argente J, Chowen JA
Frontiers in endocrinology 2018;9:168

---

MBD2-mediated Th17 differentiation in severe asthma is associated with impaired SOCS3 expression.

Sun W, Xiao B, Jia A, Qiu L, Zeng Q, Liu D, Yuan Y, Jia J, Zhang X, Xiang X
Experimental cell research 2018 Oct 1;371(1):196-204

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Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Lian S, Wang D, Xu B, Guo W, Wang L, Li W, Ji H, Wang J, Kong F, Zhen L, Li S, Zhang L, Guo J, Yang H
Behavioural brain research 2018 Jul 2;346:1-10

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The Hypothalamic Inflammatory/Gliosis Response to Neonatal Overnutrition Is Sex and Age Dependent.

Argente-Arizón P, Díaz F, Ros P, Barrios V, Tena-Sempere M, García-Segura LM, Argente J, Chowen JA
Endocrinology 2018 Jan 1;159(1):368-387

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Di-(2-ethylhexyl)-phthalate induces glucose metabolic disorder in adolescent rats.

Xu J, Zhou L, Wang S, Zhu J, Liu T, Jia Y, Sun D, Chen H, Wang Q, Xu F, Zhang Y, Liu H, Zhang T, Ye L
Environmental science and pollution research international 2018 Feb;25(4):3596-3607

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Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor-dependent signaling in human gingival epithelial cells.

Elkaim R, Bugueno-Valdebenito IM, Benkirane-Jessel N, Tenenbaum H
Journal of oral microbiology 2017;9(1):1334503

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Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment.

Fang L, Hodge J, Saaoud F, Wang J, Iwanowycz S, Wang Y, Hui Y, Evans TD, Razani B, Fan D
Oncoimmunology 2017;6(5):e1312042

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Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Igbe I, Shen XF, Jiao W, Qiang Z, Deng T, Li S, Liu WL, Liu HW, Zhang GL, Wang F
Oncotarget 2017 Dec 26;8(69):113734-113748

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6-Hydroxy-3-O-methyl-kaempferol 6-O-glucopyranoside potentiates the anti-proliferative effect of interferon α/β by promoting activation of the JAK/STAT signaling by inhibiting SOCS3 in hepatocellular carcinoma cells.

Wonganan O, He YJ, Shen XF, Wongkrajang K, Suksamrarn A, Zhang GL, Wang F
Toxicology and applied pharmacology 2017 Dec 1;336:31-39

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MiR-155 Enhances Insulin Sensitivity by Coordinated Regulation of Multiple Genes in Mice.

Lin X, Qin Y, Jia J, Lin T, Lin X, Chen L, Zeng H, Han Y, Wu L, Huang S, Wang M, Huang S, Xie R, Liang L, Liu Y, Liu R, Zhang T, Li J, Wang S, Sun P, Huang W, Yao K, Xu K, Du T, Xiao D
PLoS genetics 2016 Oct;12(10):e1006308

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Suppressors of cytokine signaling 3 is essential for FcγR-mediated inflammatory response via enhancing CCAAT/enhancer-binding protein δ transcriptional activity in macrophages.

Yan C, Liu Y, Gao H, Wang X
Experimental cell research 2015 Sep 10;337(1):120-7

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IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation.

Dong Z, Lu X, Yang Y, Zhang T, Li Y, Chai Y, Lei W, Li C, Ai L, Tai W
BMC pulmonary medicine 2015 Feb 18;15:13

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Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.

Susnik N, Sörensen-Zender I, Rong S, von Vietinghoff S, Lu X, Rubera I, Tauc M, Falk CS, Alexander WS, Melk A, Haller H, Schmitt R
Kidney international 2014 Jun;85(6):1357-68

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Kallikrein-binding protein inhibits LPS-induced TNF-α by upregulating SOCS3 expression.

Dai Z, Lu L, Yang Z, Mao Y, Lu J, Li C, Qi W, Chen Y, Yao Y, Li L, Chen S, Zhang Y, Cai W, Yang X, Gao G
Journal of cellular biochemistry 2013 May;114(5):1020-8

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TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation.

Chang CP, Su YC, Hu CW, Lei HY
Cell death and differentiation 2013 Mar;20(3):515-23

---

Role of suppressor of cytokine signaling 3 in lipid metabolism: analysis based on a phage-display human liver cDNA library.

Luo B, Zou T, Lu N, Chai F, Ye X, Wang Y, Qi Y
Biochemical and biophysical research communications 2011 Dec 9;416(1-2):39-44

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The role of microtubule-associated protein 1S in SOCS3 regulation of IL-6 signaling.

Zou T, Ouyang L, Chen L, Dong W, Qiao H, Liu Y, Qi Y
FEBS letters 2008 Dec 10;582(29):4015-22

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BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2.

Liu Y, Dong W, Chen L, Xiang R, Xiao H, De G, Wang Z, Qi Y
The Journal of biological chemistry 2004 Sep 3;279(36):37436-44

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14025-1-AP(SOCS3 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using SOCS3 antibody 14025-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 14025-1-AP(SOCS3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 14025-1-AP(SOCS3 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue