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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- 626601 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#626601, RRID:AB_2193286
- Product name
- Purified anti-SOCS3
- Antibody type
- Monoclonal
- Antigen
- Full-length SOCS3 recombinant protein
- Description
- The SOCS3 (suppressor of cytokine signaling-3) protein is a 25 kD cytoplasmic protein that contains a SOCS box and an SH2 domain. SOCS3 has been shown to be involved the negative regulation of cytokine signaling via JAK/STAT pathway. SOCS3 has been shown to inhibit IL-2, IL-3, IL-4, IL-6, erythropoietin, prolactin, and growth hormone. SOCS3 has also been shown to regulate erythropoiesis in the fetal liver. SOCS3 is induced induced by inflammation, erythropoietin, GM-CSF, IL-10, and IFN-γ. SOCS3 can be modified by JAK phophorylation which destabilizes the protein and ubiquitination which targets for destruction by the proteosome. SOCS3 has been shown to interact with the IGF-1 receptor, insulin receptor, gp130 receptor, erythropoietin receptor, leptin receptor, LIF receptor, JAK2, and elongin C. The SOC-25 monoclonal antibody recognizes human and mouse SOCS3 and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
Submitted references Serum amyloid A overrides Treg anergy via monocyte-dependent and Treg-intrinsic, SOCS3-associated pathways.
Blocking angiotensin-converting enzyme induces potent regulatory T cells and modulates TH1- and TH17-mediated autoimmunity.
Elevated expression and polymorphisms of SOCS3 influence patient response to antiviral therapy in chronic hepatitis C.
Nguyen KD, Macaubas C, Nadeau KC, Truong P, Yoon T, Lee T, Park JL, Mellins ED
Blood 2011 Apr 7;117(14):3793-8
Blood 2011 Apr 7;117(14):3793-8
Blocking angiotensin-converting enzyme induces potent regulatory T cells and modulates TH1- and TH17-mediated autoimmunity.
Platten M, Youssef S, Hur EM, Ho PP, Han MH, Lanz TV, Phillips LK, Goldstein MJ, Bhat R, Raine CS, Sobel RA, Steinman L
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 1;106(35):14948-53
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 1;106(35):14948-53
Elevated expression and polymorphisms of SOCS3 influence patient response to antiviral therapy in chronic hepatitis C.
Persico M, Capasso M, Russo R, Persico E, Crocè L, Tiribelli C, Iolascon A
Gut 2008 Apr;57(4):507-15
Gut 2008 Apr;57(4):507-15
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HepG2 lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against human SOCS3. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
