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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AP11496PU-N - Provider product page
- Provider
- OriGene
- Product name
- LIN28B rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- LIN28B rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- LIN28B
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LIN28B antibody in K562 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab (1:60 dilution).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of LIN28B antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28B antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human Spleen tissue reacted with LIN28B antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of HL-60 cells using LIN28B antibody (Center) ?(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
