24017-1-AP

antibody from Invitrogen Antibodies

Targeting: LIN28B CSDD2, FLJ16517

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24017-1-AP

Provider

Invitrogen Antibodies

Product name

LIN28B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LESIRVTGP GGSPCLGSER RPKGKTLQKR KPKGDRCYNC GGLDHHAKEC SLPPQPKKCH YCQSIMHMVA NCPHKNVAQP PASSQGRQEA ESQPCTSTLP REVGGGHGCT SPPFPQEARA EISERSGRSP QEASSTKSSI APEEQSKKGP SVQKRKKT (94-250 aa encoded by BC137526 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse embryo tissue were subjected to SDS PAGE followed by western blot with 24017-1-AP ( LIN28B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24017-1-AP (LIN28B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 24017-1-AP (LIN28B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 24017-1-AP (LIN28B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-LIN28B (IP:24017-1-AP, 4ug; Detection:24017-1-AP 1:500) with HEK-293 cells lysate 1480ug.