Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-97604 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL8A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COL8A1 using a COL8A1 Polyclonal antibody (Product # PA5-97604) at a concentration of 3 µg/mL. Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 74 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COL8A1 in U251 cells using a COL8A1 polyclonal antibody (Product # PA5-97604) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.