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H2AX antibody from Novus Biologicals
H2AFX
- Western blot
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- Immunocytochemistry
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- Immunohistochemistry
- Data presented on provider website
Antibody data
- Antibody Data
- References [27]
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- Validations
- Western blot [5]
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- Product number
- NB100-638
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-638, RRID:AB_10002048
- Product name
- Rabbit Polyclonal Histone H2AX Antibody
- Provider product page
- Novus Biologicals - NB100-638
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Autophagy role(s) in response to oncogenes and DNA replication stress.
Vanzo R, Bartkova J, Merchut-Maya JM, Hall A, Bouchal J, Dyrskjøt L, Frankel LB, Gorgoulis V, Maya-Mendoza A, Jäättelä M, Bartek J
Cell death and differentiation 2020 Mar;27(3):1134-1153
Development of CAPER peptides for the treatment of triple negative breast cancer.
Chilewski SD, Bhosale D, Dees S, Hutchinson I, Trimble R, Pontiggia L, Mercier I, Jasmin JF
Cell cycle (Georgetown, Tex.) 2020 Feb;19(4):432-447
CAPER as a therapeutic target for triple negative breast cancer.
Campbell MC, Pontiggia L, Russell AY, Schwarting R, Camacho J, Jasmin JF, Mercier I
Oncotarget 2018 Jul 13;9(54):30340-30354
The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice.
Pelz L, Purfürst B, Rathjen FG
The Journal of biological chemistry 2017 Dec 29;292(52):21490-21503
HUWE1 interacts with PCNA to alleviate replication stress.
Choe KN, Nicolae CM, Constantin D, Imamura Kawasawa Y, Delgado-Diaz MR, De S, Freire R, Smits VA, Moldovan GL
EMBO reports 2016 Jun;17(6):874-86
The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition.
Weyemi U, Redon CE, Choudhuri R, Aziz T, Maeda D, Boufraqech M, Parekh PR, Sethi TK, Kasoji M, Abrams N, Merchant A, Rajapakse VN, Bonner WM
Nature communications 2016 Feb 15;7:10711
A BRCA1-interacting lncRNA regulates homologous recombination.
Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T
EMBO reports 2015 Nov;16(11):1520-34
Numerical chromosomal instability mediates susceptibility to radiation treatment.
Bakhoum SF, Kabeche L, Wood MD, Laucius CD, Qu D, Laughney AM, Reynolds GE, Louie RJ, Phillips J, Chan DA, Zaki BI, Murnane JP, Petritsch C, Compton DA
Nature communications 2015 Jan 21;6:5990
In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation.
Yokonishi T, Sato T, Katagiri K, Komeya M, Kubota Y, Ogawa T
Biology of reproduction 2013 Jul;89(1):15
Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses.
He J, Shi LZ, Truong LN, Lu CS, Razavian N, Li Y, Negrete A, Shiloach J, Berns MW, Wu X
The Journal of biological chemistry 2012 Sep 14;287(38):31747-56
Deregulation of DNA damage response pathway by intercellular contact.
Kang MA, So EY, Ouchi T
The Journal of biological chemistry 2012 May 11;287(20):16246-55
FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response.
Xie J, Peng M, Guillemette S, Quan S, Maniatis S, Wu Y, Venkatesh A, Shaffer SA, Brosh RM Jr, Cantor SB
PLoS genetics 2012 Jul;8(7):e1002786
Contrasting DNA damage checkpoint responses in epithelium of the human seminal vesicle and prostate.
Jäämaa S, Sankila A, Rantanen V, Peltonen K, Järvinen PM, Af Hällström TM, Ruutu M, Taari K, Andersson LC, Laiho M
The Prostate 2012 Jul 1;72(10):1060-70
TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at damaged chromosomes.
Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grøfte M, Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B, Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
Cell 2012 Aug 17;150(4):697-709
MRE11 expression is predictive of cause-specific survival following radical radiotherapy for muscle-invasive bladder cancer.
Choudhury A, Nelson LD, Teo MT, Chilka S, Bhattarai S, Johnston CF, Elliott F, Lowery J, Taylor CF, Churchman M, Bentley J, Knowles MA, Harnden P, Bristow RG, Bishop DT, Kiltie AE
Cancer research 2010 Sep 15;70(18):7017-26
Proapoptotic activity of bortezomib in gastrointestinal stromal tumor cells.
Bauer S, Parry JA, Mühlenberg T, Brown MF, Seneviratne D, Chatterjee P, Chin A, Rubin BP, Kuan SF, Fletcher JA, Duensing S, Duensing A
Cancer research 2010 Jan 1;70(1):150-9
Udu deficiency activates DNA damage checkpoint.
Lim CH, Chong SW, Jiang YJ
Molecular biology of the cell 2009 Oct;20(19):4183-93
H2AX is required for cell cycle arrest via the p53/p21 pathway.
Fragkos M, Jurvansuu J, Beard P
Molecular and cellular biology 2009 May;29(10):2828-40
ATR and H2AX cooperate in maintaining genome stability under replication stress.
Chanoux RA, Yin B, Urtishak KA, Asare A, Bassing CH, Brown EJ
The Journal of biological chemistry 2009 Feb 27;284(9):5994-6003
PTEN contributes to profound PI3K/Akt signaling pathway deregulation in dystrophin-deficient dog muscle.
Feron M, Guevel L, Rouger K, Dubreil L, Arnaud MC, Ledevin M, Megeney LA, Cherel Y, Sakanyan V
The American journal of pathology 2009 Apr;174(4):1459-70
Identification of chromatin remodeling genes Arid4a and Arid4b as leukemia suppressor genes.
Wu MY, Eldin KW, Beaudet AL
Journal of the National Cancer Institute 2008 Sep 3;100(17):1247-59
Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells.
Song P, Xie Z, Wu Y, Xu J, Dong Y, Zou MH
The Journal of biological chemistry 2008 May 2;283(18):12446-55
Involvement of xeroderma pigmentosum group A (XPA) in progeria arising from defective maturation of prelamin A.
Liu Y, Wang Y, Rusinol AE, Sinensky MS, Liu J, Shell SM, Zou Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Feb;22(2):603-11
Homologous recombination is necessary for normal lymphocyte development.
Caddle LB, Hasham MG, Schott WH, Shirley BJ, Mills KD
Molecular and cellular biology 2008 Apr;28(7):2295-303
Histone H2AX is a mediator of gastrointestinal stromal tumor cell apoptosis following treatment with imatinib mesylate.
Liu Y, Tseng M, Perdreau SA, Rossi F, Antonescu C, Besmer P, Fletcher JA, Duensing S, Duensing A
Cancer research 2007 Mar 15;67(6):2685-92
Essential roles for Fe65, Alzheimer amyloid precursor-binding protein, in the cellular response to DNA damage.
Minopoli G, Stante M, Napolitano F, Telese F, Aloia L, De Felice M, Di Lauro R, Pacelli R, Brunetti A, Zambrano N, Russo T
The Journal of biological chemistry 2007 Jan 12;282(2):831-5
Heat shock induces phosphorylation of histone H2AX in mammalian cells.
Kaneko H, Igarashi K, Kataoka K, Miura M
Biochemical and biophysical research communications 2005 Mar 25;328(4):1101-6
Vanzo R, Bartkova J, Merchut-Maya JM, Hall A, Bouchal J, Dyrskjøt L, Frankel LB, Gorgoulis V, Maya-Mendoza A, Jäättelä M, Bartek J
Cell death and differentiation 2020 Mar;27(3):1134-1153
Development of CAPER peptides for the treatment of triple negative breast cancer.
Chilewski SD, Bhosale D, Dees S, Hutchinson I, Trimble R, Pontiggia L, Mercier I, Jasmin JF
Cell cycle (Georgetown, Tex.) 2020 Feb;19(4):432-447
CAPER as a therapeutic target for triple negative breast cancer.
Campbell MC, Pontiggia L, Russell AY, Schwarting R, Camacho J, Jasmin JF, Mercier I
Oncotarget 2018 Jul 13;9(54):30340-30354
The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice.
Pelz L, Purfürst B, Rathjen FG
The Journal of biological chemistry 2017 Dec 29;292(52):21490-21503
HUWE1 interacts with PCNA to alleviate replication stress.
Choe KN, Nicolae CM, Constantin D, Imamura Kawasawa Y, Delgado-Diaz MR, De S, Freire R, Smits VA, Moldovan GL
EMBO reports 2016 Jun;17(6):874-86
The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition.
Weyemi U, Redon CE, Choudhuri R, Aziz T, Maeda D, Boufraqech M, Parekh PR, Sethi TK, Kasoji M, Abrams N, Merchant A, Rajapakse VN, Bonner WM
Nature communications 2016 Feb 15;7:10711
A BRCA1-interacting lncRNA regulates homologous recombination.
Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T
EMBO reports 2015 Nov;16(11):1520-34
Numerical chromosomal instability mediates susceptibility to radiation treatment.
Bakhoum SF, Kabeche L, Wood MD, Laucius CD, Qu D, Laughney AM, Reynolds GE, Louie RJ, Phillips J, Chan DA, Zaki BI, Murnane JP, Petritsch C, Compton DA
Nature communications 2015 Jan 21;6:5990
In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation.
Yokonishi T, Sato T, Katagiri K, Komeya M, Kubota Y, Ogawa T
Biology of reproduction 2013 Jul;89(1):15
Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses.
He J, Shi LZ, Truong LN, Lu CS, Razavian N, Li Y, Negrete A, Shiloach J, Berns MW, Wu X
The Journal of biological chemistry 2012 Sep 14;287(38):31747-56
Deregulation of DNA damage response pathway by intercellular contact.
Kang MA, So EY, Ouchi T
The Journal of biological chemistry 2012 May 11;287(20):16246-55
FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response.
Xie J, Peng M, Guillemette S, Quan S, Maniatis S, Wu Y, Venkatesh A, Shaffer SA, Brosh RM Jr, Cantor SB
PLoS genetics 2012 Jul;8(7):e1002786
Contrasting DNA damage checkpoint responses in epithelium of the human seminal vesicle and prostate.
Jäämaa S, Sankila A, Rantanen V, Peltonen K, Järvinen PM, Af Hällström TM, Ruutu M, Taari K, Andersson LC, Laiho M
The Prostate 2012 Jul 1;72(10):1060-70
TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at damaged chromosomes.
Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grøfte M, Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B, Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
Cell 2012 Aug 17;150(4):697-709
MRE11 expression is predictive of cause-specific survival following radical radiotherapy for muscle-invasive bladder cancer.
Choudhury A, Nelson LD, Teo MT, Chilka S, Bhattarai S, Johnston CF, Elliott F, Lowery J, Taylor CF, Churchman M, Bentley J, Knowles MA, Harnden P, Bristow RG, Bishop DT, Kiltie AE
Cancer research 2010 Sep 15;70(18):7017-26
Proapoptotic activity of bortezomib in gastrointestinal stromal tumor cells.
Bauer S, Parry JA, Mühlenberg T, Brown MF, Seneviratne D, Chatterjee P, Chin A, Rubin BP, Kuan SF, Fletcher JA, Duensing S, Duensing A
Cancer research 2010 Jan 1;70(1):150-9
Udu deficiency activates DNA damage checkpoint.
Lim CH, Chong SW, Jiang YJ
Molecular biology of the cell 2009 Oct;20(19):4183-93
H2AX is required for cell cycle arrest via the p53/p21 pathway.
Fragkos M, Jurvansuu J, Beard P
Molecular and cellular biology 2009 May;29(10):2828-40
ATR and H2AX cooperate in maintaining genome stability under replication stress.
Chanoux RA, Yin B, Urtishak KA, Asare A, Bassing CH, Brown EJ
The Journal of biological chemistry 2009 Feb 27;284(9):5994-6003
PTEN contributes to profound PI3K/Akt signaling pathway deregulation in dystrophin-deficient dog muscle.
Feron M, Guevel L, Rouger K, Dubreil L, Arnaud MC, Ledevin M, Megeney LA, Cherel Y, Sakanyan V
The American journal of pathology 2009 Apr;174(4):1459-70
Identification of chromatin remodeling genes Arid4a and Arid4b as leukemia suppressor genes.
Wu MY, Eldin KW, Beaudet AL
Journal of the National Cancer Institute 2008 Sep 3;100(17):1247-59
Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells.
Song P, Xie Z, Wu Y, Xu J, Dong Y, Zou MH
The Journal of biological chemistry 2008 May 2;283(18):12446-55
Involvement of xeroderma pigmentosum group A (XPA) in progeria arising from defective maturation of prelamin A.
Liu Y, Wang Y, Rusinol AE, Sinensky MS, Liu J, Shell SM, Zou Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Feb;22(2):603-11
Homologous recombination is necessary for normal lymphocyte development.
Caddle LB, Hasham MG, Schott WH, Shirley BJ, Mills KD
Molecular and cellular biology 2008 Apr;28(7):2295-303
Histone H2AX is a mediator of gastrointestinal stromal tumor cell apoptosis following treatment with imatinib mesylate.
Liu Y, Tseng M, Perdreau SA, Rossi F, Antonescu C, Besmer P, Fletcher JA, Duensing S, Duensing A
Cancer research 2007 Mar 15;67(6):2685-92
Essential roles for Fe65, Alzheimer amyloid precursor-binding protein, in the cellular response to DNA damage.
Minopoli G, Stante M, Napolitano F, Telese F, Aloia L, De Felice M, Di Lauro R, Pacelli R, Brunetti A, Zambrano N, Russo T
The Journal of biological chemistry 2007 Jan 12;282(2):831-5
Heat shock induces phosphorylation of histone H2AX in mammalian cells.
Kaneko H, Igarashi K, Kataoka K, Miura M
Biochemical and biophysical research communications 2005 Mar 25;328(4):1101-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: H2AX Antibody [NB100-638] - Human 293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at the indicated concentrations.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H2AX Antibody [NB100-638] - Human 293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at the indicated concentrations.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H2AX Antibody [NB100-638] - Downregulation of the transcription factors Slug and ZEB1 reverses the EMT programme induced by H2A.X deficiency. Immunoblot analysis of EMT markers in HCT116 parental cells (WT) and H2A.X knockout cells (KO) transfected for 3 days with siRNA control (siC) or with a pool of siSLUG and siZEB1 (siS/Z) with tubulin-loading controls. H2A.X knockout cells were generated using CRISPR/Cas9 system for precise deletion of the H2A.X gene. cl.5, clone #5; and cl.6, clone #6. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms10711), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H2AX Antibody [NB100-638] - H2A.X re-expression inhibits EMT and promotes metastatic colonization in the lung. Immunoblot analysis of EMT markers in HCT116 parental cells (WT), H2A.X knockout cells (KO) and H2A.X knockout cells in which H2A.X expression was restored (KO+H2A.X), utilizing actin as a loading control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms10711), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H2AX Antibody [NB100-638] - Downregulation of the transcription factors Slug and ZEB1 reverses the EMT programme induced by H2A.X deficiency. Control cells (shCTRL) and cells silenced for H2A.X (shH2A.X) were transfected for 3 days with control siRNA (siC) or with a pool of siSLUG and siZEB1 (siS/Z). Immunoblot analysis of EMT markers was performed using tubulin as loading control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms10711), licensed under a CC-BY licence.
