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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-112307 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Applications Reported: This LN3 antibody has been reported for use in flow cytometric analysis.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SNX3 using SNX3 Polyclonal Antibody (Product # PA5-112307) at a dilution of 1:500. Lane A: HL-60 whole cell lysate, Lane B: THP1 whole cell lysate, Lane C: U-251M whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SNX3 in A431 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SNX3 Polyclonal Antibody (Product # PA5-112307) (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SNX3 in formalin-fixed paraffin embedded sections human kidney tissue sections using SNX3 Polyclonal Antibody (Product # PA5-112307) at a dilution of 1:100.
