Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 681702 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681702, RRID:AB_2566496
- Product name
- Purified anti-Siglec-11
- Antibody type
- Monoclonal
- Antigen
- Recombinant Siglec-11 fused to Fc region of human IgG.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4C4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Protein (15 µg) harvested from MCF-7, Daudi and PBMCs were resolved by electrophoresis (A) or treated with 100U PNGase F (used to cleave N-linked glycans) before electrophoresis (B), transferred to nitrocellulose, and probed with purified monoclonal Siglec-11 (clone 4C4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated