NB300-186

antibody from Novus Biologicals

Targeting: CADM1 BL2, IGSF4, IGSF4A, Necl-2, NECL2, RA175, ST17, SYNCAM, SYNCAM1, TSLC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NB300-186

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-186, RRID:AB_2121874

Product name

Rabbit Polyclonal IGSF4A/SynCAM1/CADM1 Antibody

Antibody type

Polyclonal

Description

Unpurified.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

SMAGP, a new small trans-membrane glycoprotein altered in cancer.

Tarbé NG, Rio MC, Weidle UH
Oncogene 2004 Apr 22;23(19):3395-403

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Simple Western lane view shows a specific band for SynCAM in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Rat brain and HEK cell lysates showing different glycosylation patterns. Brain fractions give banding patterns of a core glycosylation population around 50kDa and a slightly higher glycosylated population around 65 kDa. The heterologous HEK cell lysates give a single, fully glycosylated, band around 107kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IGSF4A/SynCAM1/CADM1 Antibody [NB300-186] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY CADM1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SynCAM/CADM1.