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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- AF3664 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Apolipoprotein A-I/ApoA1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P02647- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2).
Zhou G, Marathe GK, Hartiala J, Hazen SL, Allayee H, Tang WH, McIntyre TM
The Journal of biological chemistry 2013 Apr 26;288(17):11940-8
The Journal of biological chemistry 2013 Apr 26;288(17):11940-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein A-I/ApoA1 by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein A-I/ApoA1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
