NBP2-22395

antibody from Novus Biologicals

Targeting: SYT3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-22395

Provider

Novus Biologicals

Product name

Mouse Monoclonal Synaptotagmin 3 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 75kDa. Does not cross-react with other Synaptotagmins.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Synaptotagmin 3 Antibody (S278-19) [NBP2-22395] - analysis of Rat Brain Membrane showing detection of 75 kDa Synaptotagmin-3 protein using Mouse Anti-Synaptotagmin-3 Monoclonal Antibody, Clone S278-19 . Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-3 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 75 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Synaptotagmin 3 Antibody (S278-19) [NBP2-22395] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Nucleus. Magnification: 60X.