Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-56560 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NeuN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PTQDYSGQTP VPTEHGMTLY TPAQTHPEQP GSEASTQPIA GTQTVPQTDE AAQTDSQPLH PSDPTEKQQ
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NeuN Polyclonal Antibody, (Product # PA5-56560) and band at ~56 kDa corresponding to NeuN was observed across the Mouse and Rat tissues tested except Heart and Kidney which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Mouse Heart (Lane 3), Mouse Kidney (Lane 4), Rat Brain (Lane 5), Rat Cerebellum (Lane 6), Rat Heart (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NeuN in human cell line A549 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a NeuN Polyclonal Antibody (Product # PA5-56560).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NeuN in human cerebral cortex tissue shows strong cytoplasmic and nuclear positivity in neuronal cells. Samples were probed using a NeuN Polyclonal Antibody (Product # PA5-56560).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NeuN in human cerebral cortex and pancreas tissues using NeuN Polyclonal Antibody (Product # PA5-56560). Corresponding RBFOX3 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NeuN in human pancreas using NeuN Polyclonal Antibody (Product # PA5-56560) shows low expression as expected.