PA5-56560

antibody from Invitrogen Antibodies

Targeting: RBFOX3 FOX-3, HRNBP3, NeuN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56560

Provider

Invitrogen Antibodies

Product name

NeuN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PTQDYSGQTP VPTEHGMTLY TPAQTHPEQP GSEASTQPIA GTQTVPQTDE AAQTDSQPLH PSDPTEKQQ

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-NeuN Polyclonal Antibody, (Product # PA5-56560) and band at ~56 kDa corresponding to NeuN was observed across the Mouse and Rat tissues tested except Heart and Kidney which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Mouse Heart (Lane 3), Mouse Kidney (Lane 4), Rat Brain (Lane 5), Rat Cerebellum (Lane 6), Rat Heart (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NeuN in human cell line A549 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a NeuN Polyclonal Antibody (Product # PA5-56560).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NeuN in human cerebral cortex tissue shows strong cytoplasmic and nuclear positivity in neuronal cells. Samples were probed using a NeuN Polyclonal Antibody (Product # PA5-56560).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NeuN in human cerebral cortex and pancreas tissues using NeuN Polyclonal Antibody (Product # PA5-56560). Corresponding RBFOX3 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NeuN in human pancreas using NeuN Polyclonal Antibody (Product # PA5-56560) shows low expression as expected.