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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF4817 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse SOD3/EC-SOD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse SOD3/EC-SOD in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse SOD1 or SOD2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O09164- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pretreatment with statin attenuates the cardiotoxicity of Doxorubicin in mice.
Riad A, Bien S, Westermann D, Becher PM, Loya K, Landmesser U, Kroemer HK, Schultheiss HP, Tschöpe C
Cancer research 2009 Jan 15;69(2):695-9
Cancer research 2009 Jan 15;69(2):695-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse SOD3/EC-SOD by Western Blot. Western blot shows lysates of mouse lung and kidney tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Mouse SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant mouse SOD1, SOD2, and SOD3 (5 ng/lane) were included. A specific band for SOD3/EC-SOD was detected at approxi-mately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
